Reagent Kit HemeScreen® HRM Screening MPN Panel For use with HRM-enabled RT-PCR Machine

The HemeScreen® MPN Panel offerings are research use only assays that screen for somatic mutations in targets of interest related to myeloproliferative disorders

This reagent set is specifically designed to screen for mutations using High Resolution Melt Analysis in JAK2, MPL and CALR genes

This reagent set is designed for use by suitably trained personnel testing DNA extracted from whole blood or bone marrow samples using a RT-PCR-based method, or High Resolution Melt (HRM) Analysis

The HemeScreen® HRM Screening Reagents indicate the presence of a potential mutation within a sample but does not confirm the identity of the mutation; to confirm the precise mutation detected, further analysis, such as TaqMan™ detection assays or DNA sequencing, is required

The HemeScreen® MPN HRM Screening Reagents have been designed to PCR amplify the relevant regions in MPL, CALR, JAK2 exon 12, JAK2 exon 13 and JAK2 exon 14 genes for subsequent HRM analysis