Anaerobe Differentiation Disc HardyDisk™ Nitrogen

The HardyDisk™ Nitrate detects the nitrite that organisms produce by the colored end product that is formed after addition of nitrate reagents

Nitrate, when combined with sulfanilic acid and N,N-dimethyl-alpha-naphthylamine forms a red compound, p-sulfobenzene-azo-napthylamine, which is indicative of a positive reaction

If there is no color production (a negative reaction) zinc dust is added to confirm the negative reaction

HardyDisks™ Nitrate are impregnated with potassium nitrate and sodium molybdate